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Category:
Natural Sciences

Lecturer:
Dr. David Schnörr, Theoretical Systems Biology Group, Imperial College London, UK

Place:
BioQuant, Seminar room 043, Im Neuenheimer Feld 267

Host:
Jürgen Pahle, BioQuant

Description:
Cells adapt to changing conditions by changing their gene expression, both at the RNA and protein level. RNA abundances are the result of a balance between several different production and decay processes. Traditionally, most efforts have been focussed on changes in the rate of transcription. In this talk, I will use high-throughput RNA-protein binding data to try to deconvolve the effects of transcription and decay on the kinetics of RNA in yeast cells responding to nutrient shifts. I will describe how a new technology, chiCRAC, allowed us to obtain a high frequency time resolved data set of the binding to RNA of polymerase as well as two major decay factors, Nab3 and Xrn1. Using Gaussian process regression for the protein-binding data and a mechanistic model for the Rna dynamics, we find that these three proteins alone are sufficient to explain the dynamic behaviour of a surprisingly large number of transcripts during stress response. Joint work with Guido Sanguinetti, Alina Selega, Edward Wallace, Rob van Nues and Sander Granneman.

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