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Recent publication by CellNetworks junior group leader Alexis Maizel
Posted September 13 2011
Paper in The Plant Journal paves the way for exciting new discoveries in plant developmental and cell biology

The paper High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy by Alexis Maizel (CellNetworks junior group leader), Daniel von Wangenheim, Fernán Federici, Jim Haseloff and Ernst H.K. Stelzer (CellNetworks alumnus) was published in "The Plant Journal" online on 4 August 2011. The printed version will be published in October 2011.

   
 
   

In this paper the use of light-sheet based fluorescence microscopy to image with minimal invasiveness the growth of plants is described. In light-sheet microscopy, the sample is optically sectioned by a laser beam and emitted light is collected by a second objective, resulting in very low photo-toxicity, due to the use of minimal power of laser light. The key feature of the researchers' approach is that the plants grow in a environment nearly identical to the natural (lab) one. This is a major improvement over previously existing approaches in which plants grew under far-from-natural conditions.

They achieved high spatial resolution over long time periods (several days). This allowed them to uncover a new growth pattern of the root system. They also illustrate the power and versatility of the technique by recording, in the context of a whole growing organism, individual cell division as well as intra-cellular movement of organelles. This paper paves the way for exciting new discovery in plant developmental and cell biology.

The Maizel lab at the Centre for Organismal Studies Heidelberg (COS) is studying the mechanisms of developmental plasticity and robustness in the model plant Arabidopsis thaliana. This work is the result of a collaboration with the laboratory of Pr. H. K. Stelzer (Uni Frankfurt).

Please find more information about the paper here.

Go to Maizel lab website.