Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles
2014
Authors: Acuña R, Cifuentes-Muñoz N, Márquez CL, Bulling M, Klingström J, Mancini R, Lozach PY, Tischler ND
CellNetworks People: Lozach Pierre-Yves
Journal: J Virol. 2014 Feb;88(4):2344-8. doi: 10.1128/JVI.03118-13. Epub 2013 Dec 11

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.