PhD position: Maintenance of the nuclear pore complex in agfed cells and their plasticity in cancer
Category:
Phd

Description:

The nuclear pore complex (NPC) is a large cylindrical structure of over 30 different proteins named nucleoporins (NUPs) that is embedded in the nuclear envelope. It facilitates the essential process of nuclear-cytoplasmic transport of RNA molecules and proteins.

 

NPCs assemble in the intact nuclear envelope by an inside-out mechanism. How this process works, is modulated during aging and changed in cancer cells is largely unknown and will be studied in this project. We have developed a fast and highly efficient CRISPR approach for gene tagging. With this in hand, we will tag endogenous NUPs with fluorophores such as NeonGreen and degron tags and study their behaviour, function and biogenesis in different cell lines and under various growth conditions.

 

The PhD student will use biochemical approaches, conventional light and super resolution microscopy (STED; SIM) and electron microscopy for the analysis of the NPCs. CRISPR technology will be used for genomic tagging of human NUPs.

 

Highly motivated PhD students with a background in cell biology or molecular biology should apply. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research (http://www.cell.com/developmental-cell/meet-the-author/berati-cerikan). The PhD student will be a member of the Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (http://www.hbigs.uni-heidelberg.de/).

 

The PhD position (TV- L E13 (part-time)) is funded for 3 years. Please send applications to E. Schiebel (schiebel.elmar [ aT ] zmbh.uni-heidelberg.de) by 17 December 2018

 

Mailing address: Prof Elmar Schiebel, ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany

 

Please understand that received applications cannot be returned. In case of same aptitude, priority employment for handicapped people.

 

For literature on this topic, please follow this link to our website:

 

http://www.zmbh.uni-heidelberg.de/schiebel/open.html