Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins
Authors: Butkevich AN, Ta H, Ratz M, Stoldt S, Jakobs S, Belov VN, Hell SW
CellNetworks People: Hell Stefan
Journal: ACS Chem Biol. 2017 Oct 2. doi: 10.1021/acschembio.7b00616

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.