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PhD Position in Mechanistic studies of the spindle position checkpoint
Category:
Phd

Description:

In asymmetrically dividing cells, such as stem cells or budding yeast, the differential distribution of cell fate determinants and/or cellular components depends on the correct orientation of the mitotic spindle. In certain cell types, including budding yeast and Drosophila germline stem cells, spindle mis-orientation is sensed by a surveillance mechanism. In yeast, this mechanism, named as the spindle orientation checkpoint (SPOC), is essential for genome integrity and cell survival (Pereira and Yamashita, 2011; Caydasi et al., 2010, 2012).

The aim of this project is to investigate how novel SPOC components inhibit the onset of cell division in cells with a defective spindle apparatus in budding yeast. For this, the successful candidate will employ a combination of advanced microscopy, proteomic and genomic approaches to analyse protein localization, complex formation and phenotypes associated with loss- and gain-of-function mutants.

Informal inquiries should be sent to gislene.pereira [ aT ] cos.uni-heidelberg.de

References:

Caydasi, A.K. and Pereira, G. (2012). SPOC alert - when chromosomes get the wrong direction. Exp. Cell Res., 318:1421-1427.

Pereira G. and Yamashita, Y. (2011). Fly meets yeast: checking correct orientation of cell division. Trends Cell Biol., 21:526-533.

Caydasi, A.K., Ibrahim, B. and G. Pereira (2010). Monitoring spindle orientation: spindle position checkpoint in charge. Cell Div., 5:28.

Methods that will be used:

Genome wide screens, advanced microscopy (live cell imaging, FRAP, FRET), proteomic approaches in addition to standard molecular and cell biology methods.

Personal qualifications:

Applicants should have a degree in Biological Sciences with a solid background in cell and molecular biology, and special interested in research related to asymmetric cell division and cell cycle control. Applicants should be highly motivated, self-driving and able to work independently and as part of a team of international researchers.

Keywords:

Asymmetric cell division, cell cycle, yeast, centrosome, signalling, mitosis.